Biologically Relevant MicroRNA Target Prediction by Integrating MicroRNA and mRNA Expression Data using Globaltest

.................................................................................................................................................. 3 Background .............................................................................................................................................. 4 Results ..................................................................................................................................................... 7 miR-26a and EZH2 ........................................................................................................................... 7 The Combined Set of Targets for miR-26a ...................................................................................... 8 miR-1 and miR-206 .............................................................................................................................. 9 Materials and Methods ......................................................................................................................... 11 Globaltest .......................................................................................................................................... 11 Weighted Globaltest...................................................................................................................... 11 Advantages of New Approach ........................................................................................................... 12 Target Prediction selection ............................................................................................................... 12 Data structure and flow..................................................................................................................... 13 Target predictors ............................................................................................................................... 14 Expression Datasets ........................................................................................................................... 14 Software and Computational Tools ................................................................................................... 15 Discussion .............................................................................................................................................. 16 Prediction Database Selection....................................................................................................... 16 Significance of Adding Weights to Globaltest ............................................................................... 17 miRNA-target gene relations ......................................................................................................... 17 Target Prediction Database Issues ................................................................................................ 18 Target Prediction Score Processing ............................................................................................... 18 Proof of Concept and Validation Dataset Considerations ............................................................. 18 Conclusion ............................................................................................................................................. 20 References ............................................................................................................................................. 21